PD-L1 Antibody Guideline: Preparation, Dilution and Applications

PD-L1 Antibody Overview

There are 38 PD-L1 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, WB, FCM, ELISA(Cap), ELISA(Det), Neutralization, IP. There are 3 PD-L1 antibody for IHC-P, 17 PD-L1 antibody for ELISA, 2 PD-L1 antibody for WB, 16 PD-L1 antibody for FCM, 1 PD-L1 antibody for ELISA(Cap), 1 PD-L1 antibody for ELISA(Det), 1 PD-L1 antibody for Neutralization, 1 PD-L1 antibody for IP. Among all these PD-L1 antibodies, there are 12 anti-PD-L1 mouse monoclonal antibodies , 16 anti-PD-L1 rabbit monoclonal antibodies , 10 anti-PD-L1 rabbit polyclonal antibodies . All the PD-L1 anbodies are produced in house and all are in stock. PD-L1 antibody customerized service is available.

PD-L1 gene family antibody

PD-L1 Antibody ELISA

PD-L1 antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PD-L1 / B7-H1 / CD274 (rh PD-L1 / B7-H1 / CD274; Catalog#10084-H08H; NP_054862.1; Met1-Thr239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PD-L1 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

PD-L1 Antibody western blot

PD-L1 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Mouse PD-L1/B7-H1/CD274 (rM PD-L1/B7-H1/CD274; Catalog#50010-M08H; NP_068693.1; Met1-Thr238). PD-L1/B7-H1/CD274 specific IgG was purified by Mouse PD-L1/B7-H1/CD274 affinity chromatography.

PD-L1 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

PD-L1 Antibody flow cytometry

PD-L1 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PD-L1/B7-H1/CD274 (rh PD-L1/B7-H1/CD274; Catalog#10084-H08H; NP_054862.1; Met1-Thr239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PD-L1 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

PD-L1 Antibody immunohistochemistry

PD-L1 Antibody immunohistochemistry Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L1 / B7-H1 / CD274 (rh PD-L1 / B7-H1 / CD274; Catalog#10084-H08H; NP_054862.1; Met 1-Thr 239).

PD-L1 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:100-1:500
Notes  

PD-L1 Antibody immunoprecipitation

PD-L1 Antibody ICC / IF

PD-L1 Antibody Neutralization

PD-L1 antibody citations

Title
PD-L1 expression is characteristic of a subset of aggressive B-cell lymphomas and virus-associated malignancies
Year
2013
Author
BJ Chen, B Chapuy, J Ouyang, HH Sun…
Journal
Clinical Cancer research
Title
Society for Pediatric Pathology Fall Meeting, Salt Lake City, Utah, September 26–29, 2013
Year
2013
Author
C Galambos, S Sims-Lucas, AH Abman
Journal
Society for Pediatric Pathology Fall Meeting, Salt Lake City, UT
Title
PD-L1 Expression Is Increased in a Subset of Basal Type Breast Cancer Cells
Year
2014
Author
Soliman, H;Khalil, F;Antonia, S;
Journal
PLoS ONE
Title
Magnitude of PD-1, PD-L1 and T Lymphocyte Expression on Tissue from Castration-Resistant Prostate Adenocarcinoma: An Exploratory Analysis
Year
2015
Author
Massari, F;Ciccarese, C;Caliò, A;Munari, E;Cima, L;Porcaro, AB;Novella, G;Artibani, W;Sava, T;Eccher, A;Ghimenton, C;Bertoldo, F;Scarpa, A;Sperandio, N;Porta, C;Bronte, V;Chilosi, M;Bogina, G;Zamboni, G;Tortora, G;Samaratunga, H;Martignoni, G;Brunelli, M;
Journal
Target Oncol
Title
PD-L1 Antibodies to Its Cytoplasmic Domain Most Clearly Delineate Cell Membranes in Immunohistochemical Staining of Tumor Cells
Year
2015
Author
Mahoney, KM;Sun, H;Liao, X;Hua, P;Callea, M;Greenfield, EA;Hodi, FS;Sharpe, AH;Signoretti, S;Rodig, SJ;Freeman, GJ;
Journal
Cancer Immunol Res
Title
Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?
Year
2016
Author
Bæk, R;Varming, K;Jørgensen, MM;
Journal
Transfus. Apher. Sci.
Title
Developing a LANCE TR-FRET Assay for Screening PD-1 and PD-L1 Binding Inhibitors
Year
2017
Author
Hinterneder, J;Hopkinton, MA;
Journal
Product
Title
Expression of PD-L1 and other immunotherapeutic targets in thymic epithelial tumors
Year
2017
Author
Arbour, KC;Naidoo, J;Steele, KE;Ni, A;Moreira, AL;Rekhtman, N;Robbins, PB;Karakunnel, J;Rimner, A;Huang, J;Riely, GJ;Hellmann, MD;
Journal
PLoS ONE
Title
Gene signature driving invasive mucinous adenocarcinoma of the lung
Year
2017
Author
Guo, M;Tomoshige, K;Meister, M;Muley, T;Fukazawa, T;Tsuchiya, T;Karns, R;Warth, A;Fink-Baldauf, IM;Nagayasu, T;Naomoto, Y;Xu, Y;Mall, MA;Maeda, Y;
Journal
EMBO Mol Med
Title
Radiation therapy related down-regulation of PD-L1 on high-grade poorly differentiated sarcomas justify the combined radio-immunotherapy
Year
2017
Author
Wei, D;Canter, RJ;Qiu, H;Monjazeb, AM;
Journal
Abstract
Title
Immune profiles of desmoplastic small round cell tumor and synovial sarcoma suggest different immunotherapeutic susceptibility upfront compared to relapse specimens
Year
2018
Author
Wedekind, MF;Haworth, KB;Arnold, M;Stanek, JR;Lee, D;Cripe, TP;
Journal
Pediatr Blood Cancer
Title
Safety, anti-tumour activity, and pharmacokinetics of fixed-dose SHR-1210, an anti-PD-1 antibody in advanced solid tumours: a dose-escalation, phase 1 study
Year
2018
Author
Mo, H;Huang, J;Xu, J;Chen, X;Wu, D;Qu, D;Wang, X;Lan, B;Wang, X;Xu, J;Zhang, H;Chi, Y;Yang, Q;Xu, B;
Journal
Br. J. Cancer
Title
Phage display-identified PD-L1-binding peptides reinvigorate T-cell activity and inhibit tumor progression
Year
2020
Author
Gurung, S;Khan, F;Gunassekaran, G;Yoo, J;Poongkavithai Vadevoo, S;Permpoon, U;Kim, S;Kim, H;Kim, I;Han, H;Park, J;Kim, S;Lee, B;
Journal
Biomaterials
Title
Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity
Year
2020
Author
Sethuraman, SN;Singh, MP;Patil, G;Li, S;Fiering, S;Hoopes, PJ;Guha, C;Malayer, J;Ranjan, A;
Journal
Theranostics