RAGE Antibody Guideline: Preparation, Dilution and Applications

RAGE Antibody Overview

There are 11 RAGE antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA. There are 3 RAGE antibody for WB, 4 RAGE antibody for IHC-P, 8 RAGE antibody for ELISA. Among all these RAGE antibodies, there are 8 anti-RAGE rabbit polyclonal antibodies , 2 anti-RAGE mouse monoclonal antibodies , 1 anti-RAGE rabbit monoclonal antibodies . All the RAGE anbodies are produced in house and all are in stock. RAGE antibody customerized service is available.

RAGE Antibody ELISA

RAGE antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human RAGE / AGER (rh RAGE / AGER; Catalog#11629-H08H; NP_001127; Met1-Ala344). Total IgG was purified by Protein A affinity chromatography.

RAGE antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

RAGE Antibody western blot

RAGE Antibody western blot Preparation

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human RAGE/AGER, and purified by antigen affinity chromatography.

RAGE Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

RAGE Antibody immunohistochemistry

RAGE Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human RAGE/AGER (rh RAGE/AGER; Catalog#11629-H08H; NP_001127; Met1-Ala344). RAGE/AGER specific IgG was purified by Human RAGE/AGER affinity chromatography.

RAGE Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000
Notes  

RAGE Antibody immunoprecipitation

RAGE Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human RAGE/AGER (rh RAGE/AGER; Catalog#11629-H08H; NP_001127; Met1-Ala344). RAGE/AGER specific IgG was purified by Human RAGE/AGER affinity chromatography.

RAGE Antibody immunoprecipitation Validated

Application IP
Dilution 1-5 μL/mg of lysate
Notes