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ScFv single chain antibody fragment expression and production is a routine work to support antibody discovery with antibody library. ScFv protein can be readily expressed in microbial expression system such as E. coli. It can also be expressed in mammalian cells through transient transfection. Sino Biological is capable of producing ScFv single chain antibody fragment using either production systems, depending on customer's preference.
A suitable purification tag is typically added to the C-terminus of the antibody ScFv fragment protein. Commonly used purification tags are ploy-histidine tag, FLAG-tag, HA-tag, and Myc-tag. An protease cleavage size can be designed between the His-tag and the Fab to allow tag-removal after purification.
How to Submit a Request for Antibody Production Service?
If you are interested in using our antibody production services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROfirstname.lastname@example.org. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.