|qPCR Master Mix||10000+qPCR primers||10000+qPCR standards|
High amplification efficiency
Wide linear range
Excessive SYBR Green fluorescent dye is added into PCR reaction system. SYBR Green dye binding specifically to double-stranded DNA can emit fluorescence signal, but that not binding to DNA does can't. An increase of DNA products during PCR companys with an increase of fluorescence intensity and is measured at each cycle, thus allowing DNA concentrations to be quantified.
Basic principle of SYBR Green dye method
Reference gene and target gene expression analysis by SYBR Green qPCR method A:Amplification curve B:melting curve
|Qualitiative Analysis||Gene Expression Analysis|
|Relative Quantitative Analysis||Pathogen or Virus Detection|
|Absolute Quantilative Analysis||Multiple Target Genes Detection|
A successful qPCR assay requires efficient and specific amplification of the product. Both the primers and the target sequence can affect amplification efficiency and specificity. The use of PCR primers specifically designed and validated for qPCR assays with your target of interest is highly recommended. Sinobiological provide ready-to-use qEASY qPCR primer pairs and probes.
qEASY qPCR Primer Pairs are designed using SBI's proprietary primer design algorithm. They are used for SYBR Green dye-based real-time PCR and designed according to the conserved region of all variants of a specific gene. At least one primer crosses the junction of adjacent exons to avoid amplification of genomic DNA directly and effectively. Our primer pairs cover all genes from human, mouse, rat and can be widely applied to the quantitative analysis of gene expression. cDNA used as templates, a single, correct-size band is produced in SYBR Green dye-based PCR with each pair of primers. Therefore, our primers have the characteristics with high specificity, high amplification efficiency, wide linear range and uniform reaction conditions.
|qPCR primer pairs||qPCR taqman probes|
|Unique primer design||Strong conservative property|
|Strict validation process||High specificity|
|Uniform PCR conditions to save time and cost||High sensitivity|
|~100% amplification efficiency to ensure the accuracy of quantification||Strict validation process|
|Probes of high quality|
A normal qPCR experiment is completed in 2-3 weeks. Time of particular cases are in line with genes and samples detected.
Please consult us for more detailed information.
The gene expression analysis of ICAM1 in HT29 (relative quantification) by SYBR Green qPCR.
1. The IL17A protein factor stimulates HT29 cells to express ICAM1 for 24 hours, then the expression analysis of ICAM1 is performed.
2. The antibody for the IL17A protein suppresses HT29 cell2 to express ICAM1 for 24 hours, then the expression analysis of ICAM1 is performed.
Sample names: HT29(control),HT29(+IL17A, 24 h), HT29(+antibody for IL17A, 24h)
Detected genes: SDHA (reference gene) and ICAM1
Detection method: 2–∆∆CT relative quantification (SYBR Green dye method)
Test experiment report:
1. Primer quality verification
2. Report charts of original data for detected samples
Amplification curve of SDHA
Melting curve of SDHA
Amplification curve of ICAM1
Melting curve of ICAM1
3. Analysis report
Analysis results of ICAM1 expression
Graph of different ICAM1 expression