SYBR Green qPCR Method

Powerful qPCR Technology Platform: Unique Reagents and Products

qPCR Master Mix   10000+qPCR primers   10000+qPCR standards    
      High specificity
High sensitivity
High amplification efficiency
Wide linear range
Sensitive   Specific   Accurate    

SYBR Green Dye Method of qPCR

Excessive SYBR Green fluorescent dye is added into PCR reaction system. SYBR Green dye binding specifically to double-stranded DNA can emit fluorescence signal, but that not binding to DNA does can't. An increase of DNA products during PCR companys with an increase of fluorescence intensity and is measured at each cycle, thus allowing DNA concentrations to be quantified.

Basic principle of SYBR Green dye method

Reference gene and target gene expression analysis by SYBR Green qPCR method A:Amplification curve B:melting curve

SYBR Green qPCR Applications

Qualitiative Analysis Gene Expression Analysis
Relative Quantitative Analysis Pathogen or Virus Detection
Absolute Quantilative Analysis Multiple Target Genes Detection

qEASY qPCR Primer Pairs and Probes

A successful qPCR assay requires efficient and specific amplification of the product. Both the primers and the target sequence can affect amplification efficiency and specificity. The use of PCR primers specifically designed and validated for qPCR assays with your target of interest is highly recommended. Sinobiological provide ready-to-use qEASY qPCR primer pairs and probes.

qEASY qPCR Primer Pairs are designed using SBI's proprietary primer design algorithm. They are used for SYBR Green dye-based real-time PCR and designed according to the conserved region of all variants of a specific gene. At least one primer crosses the junction of adjacent exons to avoid amplification of genomic DNA directly and effectively. Our primer pairs cover all genes from human, mouse, rat and can be widely applied to the quantitative analysis of gene expression. cDNA used as templates, a single, correct-size band is produced in SYBR Green dye-based PCR with each pair of primers. Therefore, our primers have the characteristics with high specificity, high amplification efficiency, wide linear range and uniform reaction conditions.

qPCR primer pairs qPCR taqman probes
Unique primer design Strong conservative property
Strict validation process High specificity
Uniform PCR conditions to save time and cost High sensitivity
~100% amplification efficiency to ensure the accuracy of quantification Strict validation process
  Probes of high quality

SYBR Green qPCR Service Process

SYBR Green qPCR Service Time

A normal qPCR experiment is completed in 2-3 weeks. Time of particular cases are in line with genes and samples detected.

SYBR Green qPCR Service Charge

Please consult us for more detailed information.

Example

The gene expression analysis of ICAM1 in HT29 (relative quantification) by SYBR Green qPCR.

Sample description:

1. The IL17A protein factor stimulates HT29 cells to express ICAM1 for 24 hours, then the expression analysis of ICAM1 is performed.

2. The antibody for the IL17A protein suppresses HT29 cell2 to express ICAM1 for 24 hours, then the expression analysis of ICAM1 is performed.

Sample names: HT29(control),HT29(+IL17A, 24 h), HT29(+antibody for IL17A, 24h)

Detected genes: SDHA (reference gene) and ICAM1

Detection method: 2–∆∆CT relative quantification (SYBR Green dye method)

Test experiment report:
1. Primer quality verification

2. Report charts of original data for detected samples

Amplification curve of SDHA

Melting curve of SDHA

Amplification curve of ICAM1

Melting curve of ICAM1

3. Analysis report
Analysis results of ICAM1 expression

Graph of different ICAM1 expression

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Real-time PCR Service: Gene Expression Analysis by qPCR+
- SYBR Green qPCR Method
- Taqman probe qPCR Method
- Qualitative Analysis Service of Gene Expression by qPCR
- Relative Quantitative Analysis Service of Gene Expression by qPCR
- Absolute Quantitative Analysis Service of Gene Expression by qPCR
- SNP Analysis Service by qPCR
- qPCR service request