TREM-2 Matched ELISA Antibody Pair Set,Human

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TREM-2 Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
TREM-2 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human TREM-2
线性范围
54.69-3500 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1 mg/mL of mouse anti-Human TREM-2/TREM2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11084-R016)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human TREM-2/TREM2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11084-MM08)
3. Standard Each vial contains 100 ng of recombinant Human TREM-2/TREM2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3500 pg/mL is recommended.
产品概述
This TREM-2 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human TREM-2 . It contains Human TREM-2 capture antibody, Human TREM-2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human TREM-2 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

TREM-2 Matched ELISA Antibody Pair Set,Human 图片

TREM-2 Matched ELISA Antibody Pair Set,Human: 别称

TREM-2 Matched ELISA Antibody Pair Set, Human; Trem2a Matched ELISA Antibody Pair Set, Human; Trem2b Matched ELISA Antibody Pair Set, Human; Trem2c Matched ELISA Antibody Pair Set, Human

TREM-2 背景信息

Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.
全称
triggering receptor expressed on myeloid cells 2
参考文献
  • Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995.
  • Paloneva, J. et al., 2002, Am. J. Hum. Genet. 71:656-662. 
  • Prada, I. et al., 2006, Neuroscience. 140 (4): 1139-48.
  • Neumann, H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9.
  • Thrash, JC. et al., 2009, Neurochem Res. 34 (1): 38-45.
  • TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis
    Author
    G Kleinberger, Y Yamanishi…
    Year
    2014
    Journal
    Science Translational Medicine
    Application
    ELISA
  • Role of TREM-1 in pulmonary tuberculosis patients- analysis of serum soluble TREM-1 levels
    Author
    Feng, JY;Su, WJ;Pan, SW;Yeh, YC;Lin, YY;Chen, NJ;
    Year
    2018
    Journal
    Sci Rep
    Application
    ELISA (serum sample)
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